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codon optimized peptide expression sequences  (Twist Bioscience)


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    Structured Review

    Twist Bioscience codon optimized peptide expression sequences
    Codon Optimized Peptide Expression Sequences, supplied by Twist Bioscience, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/codon/codon+experiments+expression+klhdc3+mouse+mouse+optimised+re/pm42264739-122-1-8
    Average 86 stars, based on 1 article reviews
    codon optimized peptide expression sequences - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity
    Article Snippet: Gradients were manually fractionated into 200 μL fractions and analysed using 4–15% Criterion Stain-free Midi gels (BioRad) with stain-free imaging or WB (TransBlot Turbo BioRad) against the APOBEC His-tag. .. Codon-optimized full-length human HUWE1 (Twist Bioscience) was cloned into the pMEPI vector with an N-terminal 6x-His tag, a 3 °C protease cleavage site, and a C-terminal double Strep-tag. ..

    Article Title: Monoclonal neutralizing antibodies elicited by infection with Kaposi sarcoma-associated herpesvirus reveal critical sites of vulnerability on gH/gL
    Article Snippet: .. DNA corresponding to amino acids 22–698 of KSHV gH (NCBI: YP_001129375.1 ) preceded by a TPA leader sequence (MDAMKRGLCCVLLLCGAVFVSPSAS) and followed by a GSGSG linker, a hexa-His tag and an Avi tag were codon optimized, synthesized by Twist biosciences and cloned into pTwist-CMV-OriP to create pTwist-CMV-OriP-KSHV-gH. .. DNA corresponding to amino acids 21–167 of KSHV gL (NCBI: YP_001129399.1 ) preceded by a TPA leader sequence were codon optimized, synthesized by Twist biosciences and cloned into pTwist-CMV-OriP to create pTwist-CMV-OriP-KSHV-gL.

    Article Title: ERCC6L2 ensures repair fidelity for staggered-end DNA double-strand breaks
    Article Snippet: .. The human ERCC6L2 sequence, codon optimized for the expression in Sf 9 cells, was purchased from Twist Bioscience (Supplementary Table ) and cloned into pFB-2XMBP-BRCA1co-his using NheI and XhoI and standard procedures to generate pFB_2xMBP-ERCC6L2co_his (Supplementary Table ). .. The K154A mutation was generated using QuikChange XL II kit (Agilent).

    Article Title: ERCC6L2 ensures repair fidelity for staggered-end DNA double-strand breaks.
    Article Snippet: .. The human ERCC6L2 sequence, codon optimized for the expression in Sf9 cells, was purchased from Twist Bioscience (Supplementary Table 2) and cloned into pFB-2XMBP-BRCA1co-his 76 using NheI and XhoI and standard procedures to generate pFB_2xMBP-ERCC6L2co_his (Supplementary Table 3). ..

    Article Title: Plant Kelch phosphatases are Ser/Thr phosphatases involved in cell cycle regulation
    Article Snippet: .. The Kelch domain (residues 1–357; BSU1 1-357 ) from Arabidopsis thaliana BSU1 (TAIR-ID: At1g03445, https://www.arabidopsis.org ) was cloned from codon-optimized synthetic genes (Twist Bioscience) into the pBB3 vector for expression in Spodoptera frugiperda Sf9 cells. ..

    Strep-tag:

    Article Title: Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity
    Article Snippet: Gradients were manually fractionated into 200 μL fractions and analysed using 4–15% Criterion Stain-free Midi gels (BioRad) with stain-free imaging or WB (TransBlot Turbo BioRad) against the APOBEC His-tag. .. Codon-optimized full-length human HUWE1 (Twist Bioscience) was cloned into the pMEPI vector with an N-terminal 6x-His tag, a 3 °C protease cleavage site, and a C-terminal double Strep-tag. ..

    Expressing:

    Article Title: Long-range allosteric communication, double mutant cycles, and energetic coupling in SARS-CoV-2 spike protein
    Article Snippet: .. All sequences were codon optimized by Twist Biosciences for expression in mammalian cells. ..

    Article Title: ERCC6L2 ensures repair fidelity for staggered-end DNA double-strand breaks
    Article Snippet: .. The human ERCC6L2 sequence, codon optimized for the expression in Sf 9 cells, was purchased from Twist Bioscience (Supplementary Table ) and cloned into pFB-2XMBP-BRCA1co-his using NheI and XhoI and standard procedures to generate pFB_2xMBP-ERCC6L2co_his (Supplementary Table ). .. The K154A mutation was generated using QuikChange XL II kit (Agilent).

    Article Title: ERCC6L2 ensures repair fidelity for staggered-end DNA double-strand breaks.
    Article Snippet: .. The human ERCC6L2 sequence, codon optimized for the expression in Sf9 cells, was purchased from Twist Bioscience (Supplementary Table 2) and cloned into pFB-2XMBP-BRCA1co-his 76 using NheI and XhoI and standard procedures to generate pFB_2xMBP-ERCC6L2co_his (Supplementary Table 3). ..

    Article Title: Plant Kelch phosphatases are Ser/Thr phosphatases involved in cell cycle regulation
    Article Snippet: .. The Kelch domain (residues 1–357; BSU1 1-357 ) from Arabidopsis thaliana BSU1 (TAIR-ID: At1g03445, https://www.arabidopsis.org ) was cloned from codon-optimized synthetic genes (Twist Bioscience) into the pBB3 vector for expression in Spodoptera frugiperda Sf9 cells. ..

    Article Title: Structural investigations of the glucan water dikinase 1 mechanism and flexibility
    Article Snippet: .. Sequence of StGWD1 (Genbank Accession number Q9AWA5) without its signal peptide (aa 1-77) was codon-optimized for an expression in E. coli and ordered from TWIST bioscience (San Francisco, USA). ..

    Sequencing:

    Article Title: Monoclonal neutralizing antibodies elicited by infection with Kaposi sarcoma-associated herpesvirus reveal critical sites of vulnerability on gH/gL
    Article Snippet: .. DNA corresponding to amino acids 22–698 of KSHV gH (NCBI: YP_001129375.1 ) preceded by a TPA leader sequence (MDAMKRGLCCVLLLCGAVFVSPSAS) and followed by a GSGSG linker, a hexa-His tag and an Avi tag were codon optimized, synthesized by Twist biosciences and cloned into pTwist-CMV-OriP to create pTwist-CMV-OriP-KSHV-gH. .. DNA corresponding to amino acids 21–167 of KSHV gL (NCBI: YP_001129399.1 ) preceded by a TPA leader sequence were codon optimized, synthesized by Twist biosciences and cloned into pTwist-CMV-OriP to create pTwist-CMV-OriP-KSHV-gL.

    Article Title: ERCC6L2 ensures repair fidelity for staggered-end DNA double-strand breaks
    Article Snippet: .. The human ERCC6L2 sequence, codon optimized for the expression in Sf 9 cells, was purchased from Twist Bioscience (Supplementary Table ) and cloned into pFB-2XMBP-BRCA1co-his using NheI and XhoI and standard procedures to generate pFB_2xMBP-ERCC6L2co_his (Supplementary Table ). .. The K154A mutation was generated using QuikChange XL II kit (Agilent).

    Article Title: ERCC6L2 ensures repair fidelity for staggered-end DNA double-strand breaks.
    Article Snippet: .. The human ERCC6L2 sequence, codon optimized for the expression in Sf9 cells, was purchased from Twist Bioscience (Supplementary Table 2) and cloned into pFB-2XMBP-BRCA1co-his 76 using NheI and XhoI and standard procedures to generate pFB_2xMBP-ERCC6L2co_his (Supplementary Table 3). ..

    Article Title: Structural investigations of the glucan water dikinase 1 mechanism and flexibility
    Article Snippet: .. Sequence of StGWD1 (Genbank Accession number Q9AWA5) without its signal peptide (aa 1-77) was codon-optimized for an expression in E. coli and ordered from TWIST bioscience (San Francisco, USA). ..

    Synthesized:

    Article Title: Monoclonal neutralizing antibodies elicited by infection with Kaposi sarcoma-associated herpesvirus reveal critical sites of vulnerability on gH/gL
    Article Snippet: .. DNA corresponding to amino acids 22–698 of KSHV gH (NCBI: YP_001129375.1 ) preceded by a TPA leader sequence (MDAMKRGLCCVLLLCGAVFVSPSAS) and followed by a GSGSG linker, a hexa-His tag and an Avi tag were codon optimized, synthesized by Twist biosciences and cloned into pTwist-CMV-OriP to create pTwist-CMV-OriP-KSHV-gH. .. DNA corresponding to amino acids 21–167 of KSHV gL (NCBI: YP_001129399.1 ) preceded by a TPA leader sequence were codon optimized, synthesized by Twist biosciences and cloned into pTwist-CMV-OriP to create pTwist-CMV-OriP-KSHV-gL.

    Article Title: Bio-based oxalic acid production in Issatchenkia orientalis enables sustainable rare earth recovery
    Article Snippet: S. cerevisiae YSG50 and I. orientalis SD108 were propagated in Yeast-Peptone-Dextrose (YPD) medium consisting of 1% yeast extract, 2% peptone, and 2% glucose. .. Genes were codon optimized and synthesized by Twist Bioscience (San Francisco, CA). .. LB broth, bacteriological grade agar, yeast extract, peptone, yeast nitrogen base (w/o amino acid and ammonium sulfate), and ammonium sulfate were obtained from Difco (BD, Sparks, MD), while synthetic complete supplement mixture of amino acids without uracil was obtained from MP Biomedicals (Solon, OH).

    Plasmid Preparation:

    Article Title: Plant Kelch phosphatases are Ser/Thr phosphatases involved in cell cycle regulation
    Article Snippet: .. The Kelch domain (residues 1–357; BSU1 1-357 ) from Arabidopsis thaliana BSU1 (TAIR-ID: At1g03445, https://www.arabidopsis.org ) was cloned from codon-optimized synthetic genes (Twist Bioscience) into the pBB3 vector for expression in Spodoptera frugiperda Sf9 cells. ..



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