Journal: bioRxiv
Article Title: A CandiChrome toolkit for multicolor labeling of Candida cells
doi: 10.64898/2026.05.11.723596
Figure Lengend Snippet: Cells were grown overnight in SCD at 30 °C before analysis. Fluorescence microscopy images were acquired on an Evident APEXVIEW APX100 microscope using the GFP or Cy3 imaging channels, as indicated for each panel. Flow cytometry data were acquired on a Cytek Aurora flow cytometer equipped with UV, violet, blue, and red lasers. a, Representative DIC and fluorescence images of strains expressing Eno1-mNeonGreen, Eno1-mEGFP, or Eno1-mStayGold are shown (left). Single-cell average pixel intensity was quantified using CellProfiler from at least 200 cells per strain (right). Error bars represent s.d.; Rel. units, relative units. b, Photostability was examined by repeated 1 s exposures at 30% light intensity on an Evident APX100 microscope using the GFP imaging channel. Fluorescence intensity values (left) and values normalized to T = 0 (right) are shown. Average pixel intensity was measured from at least 200 cells per strain in a representative field of view. Error bars represent s.d.; Rel. units, relative units. c, Histograms show fluorescence intensity distributions of 100,000 events acquired on an Aurora flow cytometer, with fluorescence detected in the 508 nm emission channel. Mean fluorescence intensity values are indicated below the histogram. d, Representative DIC and fluorescence images of strains expressing Eno1-dTomato, Eno1-mScarlet-I, Eno1-mScarlet3-S2 or Eno1-mKate2 are shown (left). Fluorescence images were acquired on an Evident APX100 microscope using the Cy3 channel. Single-cell average pixel intensity was quantified using CellProfiler from at least 200 cells per strain (right). Error bars represent s.d.; Rel. units, relative units. e, Cells were photobleached on an Evident APX100 microscope in the Cy3 channel by repeated 1 s exposures at 30% light intensity. Fluorescence intensity values (left) and values normalized to T = 0 (right) are shown. Average pixel intensity was measured from at least 200 cells per strain in a representative field of view. Error bars represent s.d.; Rel. units, relative units. f, Histograms show fluorescence intensity distributions of 100,000 events acquired on an Aurora flow cytometer and detected in the 581 nm and 615 nm emission channels. Mean fluorescence intensity values are indicated below the histograms.
Article Snippet: C. albicans codon-optimized mStayGold and mEGFP were synthesized by BioBasic.
Techniques: Fluorescence, Microscopy, Imaging, Flow Cytometry, Expressing, Single Cell
